Cap-Clip™ Acid Pyrophosphatase C-CC15011H 100 units
产品简介:北京中北林格供应Cap-Clip™ Acid Pyrophosphatase C-CC15011H 100 units,中北林格是cellscript总代理,提供cellscript产品货号报价查询。其他品牌包括LGC Biosearch Technologies总代理,epicentre总代理,lucigen总代理,illumina总代理,ICLLAB总代理,ImmunoReagents总代理。</p>
Cap-Clip™ Acid Pyrophosphatase C-CC15011H 100 units 产品参数
名称:Cap-Clip™ Acid Pyrophosphatase</p>
分类:mRNA Decapping</p>
货号:C-CC15011H</p>
规格:100 units</p>
产品详情:For the removal of cap structures (decapping) from various RNAs (i.e. mRNAs) resulting in 5'-monophosphate RNAs. Can be substituted unit for unit, into any preexisting protocol for Tobacco Acid Pyrophosphatase (TAP) utilizing the same buffer.</p>
供应商:中北林格</p>
产地:us</p>
发货地:北京</p>
运输方式:送货上门/顺丰</p>
是否现货:期货</p>
Cap-Clip™ Acid Pyrophosphatase C-CC15011H 100 units 英文说明书(PDF)
INTRODUCTION Cap-Clip™ Acid Pyrophosphatase was developed as an improvement over Tobacco Acid Pyrophosphatase (TAP). Cap-Clip Acid Pyrophosphatase can be used unit for unit in any previously developed protocol that utilized TAP. The Reactions Buffers for the two enzymes are identical. Cap-Clip Acid Pyrophosphatase is a plant-derived enzyme that hydrolyzes various pyrophosphate bonds, including the pyrophosphate bonds of the 5'-terminal m7 GpppG "cap" of eukaryotic messenger RNAs, as well as 5' cap structures on many small nuclear RNAs (snRNAs), heterogeneous nuclear RNAs and some viral RNAs. Complete hydrolysis of such capped RNAs generates RNA that has a 5'-monophosphate group. With lot to lot consistency, absence of critical contaminants and a dependable inventory, Cap-Clip Acid Pyrophosphatase replaces all TAP needs.
Store at –20o C in a freezer without a defrost cycle. Do not store at –70o C.
Cap-Clip™ Acid Pyrophosphatase (100 units) |
Component | Volume |
Cap-Clip™ Acid Pyrophosphatase, 5 U/μl in 50% glycerol, 10 mM Tris-HCl, pH 7.5, 100 mM NaCl, 1 mM dithiothreitol, 0.1 mM EDTA and 0.01% Triton® X-100. | 20 μl |
10X Cap-Clip™ Acid Pyrophosphatase Reaction Buffer 0.5 M NaOAc, pH 6.0, 10 mM EDTA, 1% -mercaptoethanol and 0.1% Triton X-100. | 250 μ |
SPECIFICATIONS Unit Definition One unit of Cap-Clip Acid Pyrophosphatase releases one nanomole of inorganic phosphate from m7 GpppG in 30 minutes at 37o C under standard assay conditions. Contaminating Activity Assays Cap-Clip Acid Pyrophosphatase is free of detectable RNase, DNase and phosphatase activities.
cellscript总代理中北林格 C-CC15011H 100 units</p>
cellscript mRNA Decapping C-CC15011H</p>
cellscript总代理 mRNA Decapping C-CC15011H</p>
cellscript总代理中北林格 mRNA Decapping C-CC15011H</p>
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